中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (50): 9321-9325.doi: 10.3969/j.issn.2095-4344.2012.50.003

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

双极射频不同时间治疗关节软骨损伤的后期效应

吉先群1, 2，王大平2，熊建义2，刘建全2，朱伟民2，许美权2，张晓丽3   

  • 出版日期:2012-12-09 发布日期:2012-12-09

Post-effects of bipolar radiofrequency energy treatment time on articular cartilage injury  

Ji Xian-qun1, 2, Wang Da-ping2, Xiong Jian-yi2, Liu Jian-quan2, Zhu Wei-min2, Xu Mei-quan2, Zhang Xiao-li3   

  • Online:2012-12-09 Published:2012-12-09

PDF

412

被引次数

3

摘要：

背景：射频已用于治疗软骨损伤，但其安全性及后期疗效并不确切。
目的：模拟关节镜条件下使用双极射频能量治疗关节软骨损伤，分别通过流式细胞仪和扫描电镜观察评估软骨细胞的活力和表面轮廓，进而评估双极射频治疗软骨损伤的效果及安全性。
方法：36只新西兰大白兔采用前十字韧带切断和内侧半月板切除制作双侧股骨软骨继发性损伤的动物模型。造模6周后按治疗时间不同对左侧膝关节软骨损伤行双极射频消融治疗5，10，20 s，右侧膝关节为对照侧，不做射频消融处理。治疗8周后观察双极射频治疗软骨损伤的后期效应。
结果与结论：大体观察发现，大部分双极射频处理面较对照侧软骨面光滑；10 s组和20 s组扫描电镜评分均明显高于5 s组和对照组，差异有显著性意义(P < 0.05)；20 s组细胞死亡率高于5 s组、10 s组和对照侧，差异有显著性意义(P < 0.05)，5 s组、10 s组与对照侧之间差异无显著性意义(P > 0.05)。可见双极射频治疗软骨损伤可减缓软骨的退变速度，在达到治疗效果的情况下应尽量减少治疗时间，以减少对软骨细胞损伤。

Abstract:

BACKGROUND: Radiofrequency has been used for the treatment of cartilage injury, but its safety and post-effect is not exact.
OBJECTIVE: To observe the chondrocyte viability and surface contouring through flow cytometer and scanning electron microscope respectively during different treatment periods with bipolar radiofrequency energy under simulated arthroscopy in order to evaluate the effect and safety of bipolar radiofrequency for the treatment of cartilage injury.
METHODS: Thirty-six New Zealand white rabbits were selected, and under sterile conditions, the anterior cruciate ligament removal and medial meniscectomy were performed to make models of cartilage injury. At 6 weeks after modeling, cartilage injury of the left knee joint was treated with bipolar radiofrequency for different time periods: 5, 10 and 20 seconds, the right side was as control without radiofrequency ablation. Then, the post-effect of bipolar radiofrequency for the treatment of cartilage injury was observed after treated for 8 weeks.
RESULTS AND CONCLUSION: General observation showed that the macro-pathologic articular cartilage surface of the treatment groups was smoother than that in the control group; the scanning electron microscope score in the 10 seconds group and 20 seconds group was higher than that in the 5 seconds group and control group, and the difference was significant (P < 0.05); the mortality rate of chondrocytes in 20 seconds group was significantly higher than that in 5 seconds group, 10 seconds group and the control group, and the difference was significant (P < 0.05), and there was no significant difference between 5 seconds group, 10 seconds group and control group (P > 0.05) . The treatment time should be minimized when the bipolar radiofrequency can achieve the desired effect on the treatment of articular cartilage injury, in order to reduce the damage on the chondrocytes, as the speed of cartilage degeneration can be delayed by bipolar radiofrequency treatment.